Glucocorticoid ReceptorEndocrine disruption: Estimation of the binding affinity of small molecules towards the Glucocorticoid receptor.
The glucocorticoid receptor is a member of the nuclear receptor superfamily that affects immune response, development, and metabolism in target tissues. Glucocorticoids are widely used to treat diverse pathological conditions but their clinical use is still limited by side effects. A prediction of the binding affinity towards the glucocorticoid receptor would therefore be beneficial for the design of new glucocorticoids with minimal adverse side effects, but also for identifying possible glucocorticoid-mediated toxic mechanisms triggered by drugs or chemicals. By combining flexible docking (software Yeti) to the glucocorticoid receptor and multi-dimensional QSAR (software Quasar), we validated a model based on 110 compounds, representing four different chemical classes. The good correlation with experimental data (cross-validated r² = 0.702, predictive r² = 0.719) suggests that our approach is suitable for predicting the binding affinity of novel compounds towards the glucocorticoid receptor. The model was challenged by a series of scramble tests, a consensus approach using the Raptor software as well as the prediction of eight external compounds.
Binding of R-Lorazepam to the glucocorticoid receptor (stereo view).
Comparison of experimental and predicted Ki values for the glucocorticoid receptor
(training set = black, test set = red).